Illumina 測(cè)序組合試劑盒 20005614

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Illumina 測(cè)序組合試劑盒 20005614

產(chǎn)品編號(hào)： 20005614   美  元  價(jià)： $3200.00

會(huì)  員  價(jià)： 待定     品       牌： Illumina

產(chǎn)       地： 美國(guó)    公       司： Illumina, Inc.

產(chǎn)品分類(lèi)： 分子類(lèi)>二代測(cè)序>DNA文庫(kù)制備試劑盒公司分類(lèi)： Library Preparation Kits "

Product Highlights:

TruSeq Small RNA Library Preparation Kits provide reagents to generate small RNA libraries directly from total RNA. MicroRNAs (miRNAs) generated by Dicer processing are efficiently targeted by the included modified adapters.

These kits enable multiplexed sequencing with the introduction of 48 unique indexes, allowing miRNA and small RNA discovery and profiling throughput to match the unparalleled output of Illumina sequencing. Indexes are added in a universal amplification reaction, greatly reducing ligation bias and ensuring accurate measurement of miRNA expression.

Workflow improvements enable streamlined sample preparation, allowing economical studies covering all small RNA transcripts in any species. CompatIBLe applications include finding novel miRNAs, characterizing variation such as isomers with single-base resolution, and analyzing differential expression without prior assumptions.

TruSeq Small RNA Sample Datasets

The Human Brain Reference RNA (HBRR) and Universal Human Reference RNA (UHRR) samples were prepared using the TruSeq Small RNA Library Prep Kit. These libraries were sequenced on the MiniSeq System using the MiniSeq High Output Reagent Kit at a 1 x 36 bp read length configuration. The total yield was 0.91 Gb with 97% of bases at or above Q30.

Browse the Data In BaseSpace Sequence Hub:

View Run (primary analysis and metrics)

View Project (secondary analysis with BaseSpace Apps)

Specifications:

Assay Time ~10.5 hours

Hands-On Time ~30 minutes

Input Quantity 25 to 100 ng total RNA from species with polyA tails

Content Specifications Captures the coding transcriptome with strand information

Mechanism of Action Oligo-dT beads capture polyA tails

Multiplexing Up to 24-plex pooling with additional NeoPrep runs

Sample Throughput Up to 16 samples per NeoPrep library card

Species Category Drosophila,Any Species,Mouse,Zebrafish,Human,Rat,Plant,Nematode

System Compatibility MiSeq,NextSeq 550,MiniSeq,NextSeq 500

Specialized Sample Types FFPE,Low Input

Technology Sequencing

Automation CapABIlity Liquid Handling Robots

Variant Class Novel Transcripts,Transcript Variants

Method miRNA and Small RNA Sequencing